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Extracting DNA from a Strawberry
Grade Level:
12
Subject:
Biology
Topic:
Strand D: Molecular Genetics- DNA Extraction
Objective:
Students will investigate laboratory techniques that will allow them to extract DNA from strawberries. They will be able to study cellular components at a molecular level, in order to build their understanding of genetics. This lesson is relevant to students because they will be able to physically observe the properties and structure of DNA. They will also be able to explain why DNA extraction is important to scientists and its applications.
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Prep:
Prepare the DNA extraction buffer. In a container, add 360mL of water, 40mL of dishwashing detergent (or 100mL shampoo), and finally 2 teaspoons of salt. Slowly invert the bottle to mix the extraction buffer. Bring materials and photocopy enough handouts for all of the students.
Materials:
For each student: lab goggles, lab handouts, heavy duty ziploc bag (freezer or storage bag); 1-2 strawberries; DNA extraction buffer (900mL water, 50mL dishwashing detergent, 2 teaspoons salt); small plastic cup to hold extraction buffer; cheesecloth to fit in small funnel (4” X 4” should be appropriate); small funnel; 50mL vial / test tube; glass rod, inoculating loop, or popsicle stick; cold ethanol (or isopropyl alcohol), ice.
