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Grade Level:
12
Subject:
Biology
Topic:
Strand D: Molecular Genetics- DNA Extraction
Objective:
Students will observe first hand that DNA is in the food that they eat. Students will learn the simple method to extract DNA and why each step is necessary due to the complex organization of DNA in cells.
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Prep:
Prepare the DNA extraction buffer. In a container, add 900mL of water, 50mL of dishwashing detergent (or 100mL shampoo), and finally 2 teaspoons of salt. Slowly invert the bottle to mix the extraction buffer.
Materials:
For each student: heavy duty ziploc bag (freezer or storage bag); 1-2 strawberries; DNA extraction buffer (900mL water, 50mL dishwashing detergent, 2 teaspoons salt); small plastic cup to hold extraction buffer; cheesecloth to fit in small funnel (4” X 4” should be appropriate); small funnel; 50mL vial / test tube; glass rod, inoculating loop, or popsicle stick; cold ethanol (or isopropyl alcohol), ice.
